Limits of homology detection by pairwise sequence comparison

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چکیده

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Limits of homology detection by pairwise sequence comparison

MOTIVATION Noise in database searches resulting from random sequence similarities increases as the databases expand rapidly. The noise problems are not a technical shortcoming of the database search programs, but a logical consequence of the idea of homology searches. The effect can be observed in simulation experiments. RESULTS We have investigated noise levels in pairwise alignment based da...

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The function of an unknown biological sequence can often be accurately inferred by identifying sequences homologous to the original sequence. Given a query set of known homologs, there exist at least three general classes of techniques for finding additional homologs: pairwise sequence comparisons, motif analysis, and hidden Markov modeling. Pairwise sequence comparisons are typically employed ...

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Weak Homology Detection by Profile-Profile Comparison

Experimentally determined protein tertiary structures are currently increasing in an enormous rate. The growth of the structure database makes structure prediction methods based on known tertiary structures more efficient. Detecting weak homology is crucial to extend applicable area of these methods. For this purpose, a profile or PSSM (position-specific score matrix) derived from multiple alig...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2001

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/17.4.338